4USH
Nitrogen regulatory protein PII from Chlamydomonas reinhardtii in unliganded state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.980, 89.780, 45.940 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 37.790 - 1.600 |
| R-factor | 0.17173 |
| Rwork | 0.170 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xzw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 44312 | |
| <I/σ(I)> | 13 | 1.81 |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 4.64 | 4.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M (NH4)2SO4, 0.1 M HEPES, PH 7.0, 20% (W/V) PEG 4000 |
