4US2
The crystal structure of H-Ras and SOS in complex with ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 149.710, 149.710, 200.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.930 - 2.480 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | MOSFLM |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.930 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.120 | 0.760 |
| Number of reflections | 39233 | |
| <I/σ(I)> | 11.6 | 2.5 |
| Completeness [%] | 97.3 | 99 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
