4URX

The crystal structure of H-Ras and SOS in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Collection date	2010-07-02
Spacegroup name	I 4 2 2
Unit cell lengths	149.874, 149.874, 199.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.940 - 2.490
R-factor	0.1954
Rwork	0.194
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkd
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	MOSFLM
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.870	2.620
High resolution limit [Å]	2.480	2.480
Rmerge	0.060	0.510
Number of reflections	40264
<I/σ(I)>	33.4	5.1
Completeness [%]	100.0	100
Redundancy	12	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1