4URV
The crystal structure of H-Ras and SOS in complex with ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 149.900, 149.900, 200.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.480 - 2.580 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.050 | 2.670 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.090 | 0.750 |
| Number of reflections | 35041 | |
| <I/σ(I)> | 15.9 | 2.5 |
| Completeness [%] | 97.6 | 99 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2M SODIUM FORMATE, 2% DMSO |
