4URO
Crystal Structure of Staph GyraseB 24kDa in complex with Novobiocin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS |
| Synchrotron site | ALS |
| Temperature [K] | 293 |
| Collection date | 2009-10-17 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.280, 68.590, 85.720 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 74.240 - 2.590 |
| R-factor | 0.1978 |
| Rwork | 0.195 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aj6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.240 | 2.890 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.090 | 0.350 |
| Number of reflections | 26992 | |
| <I/σ(I)> | 9.9 | 3.3 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M MAGNESIUM CHLORIDE, 0.1M TRIS PH8.5, 30% PEG 3350 |
