4URN
Crystal Structure of Staph ParE 24kDa in complex with Novobiocin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2008-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.678, 75.321, 76.144 |
| Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
| Resolution | 19.470 - 2.300 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s14 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.320 |
| Number of reflections | 28285 | |
| <I/σ(I)> | 10.3 | 3.46 |
| Completeness [%] | 97.0 | 98.4 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.15M AMMONIUM ACETATE, 0.075M TRIS PH 8.5 AND 19% PEG 3350 |
