4URJ
Crystal structure of human BJ-TSA-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.120, 50.730, 120.290 |
| Unit cell angles | 90.00, 105.27, 90.00 |
Refinement procedure
| Resolution | 42.084 - 2.680 |
| R-factor | 0.2349 |
| Rwork | 0.232 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.744 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | Rosetta (MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.080 | 2.810 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.200 | 0.600 |
| Number of reflections | 25987 | |
| <I/σ(I)> | 3.4 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8M SODIUM PHOSPHATE MONOBASIC, 0.8M SODIUM PHOSPHATE DIBASIC, 0.1M HEPES PH 7.5 |
