4URI
Crystal structure of chitinase-like agglutinin RobpsCRA from Robinia pseudoacacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | MARRESEARCH MAR25 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.100, 53.221, 71.271 |
| Unit cell angles | 106.01, 94.12, 107.59 |
Refinement procedure
| Resolution | 34.480 - 1.850 |
| R-factor | 0.15038 |
| Rwork | 0.148 |
| R-free | 0.18731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL GENERATED BY PHYRE SERVER BASED ON PDB ENTRY 1NWU |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 47466 | |
| <I/σ(I)> | 15.5 | 3.5 |
| Completeness [%] | 97.3 | 96.1 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 35,8% (V/V) MPD, 0.1 M NA/K2 PHOSPHATE PH 7.0 |
