4UQY
Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-03 |
| Detector | ADSC ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.220, 63.970, 94.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.298 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 36309 | |
| <I/σ(I)> | 10.7 | 2.2 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50 MM MES (PH 6.5) 100 MM AMMONIUM ACETATE 10% GLYCEROL 28% PEG 8000 |
