4UQA
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.732, 50.366, 58.871 |
| Unit cell angles | 65.00, 67.28, 76.84 |
Refinement procedure
| Resolution | 50.390 - 1.520 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.390 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.040 | 0.140 |
| Number of reflections | 66458 | |
| <I/σ(I)> | 23 | 10.22 |
| Completeness [%] | 96.5 | 94.6 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL 8.5, 8 % (W/V) PEG 8 K |
