4UQ9
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.77 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.620, 50.330, 58.710 |
| Unit cell angles | 65.16, 67.56, 76.99 |
Refinement procedure
| Resolution | 45.530 - 1.770 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.838 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.530 | 1.840 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.130 | 0.950 |
| Number of reflections | 40534 | |
| <I/σ(I)> | 14.15 | 1.91 |
| Completeness [%] | 93.4 | 79.5 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL 8.5, 8 % PEG 8000 |
