4UPA
Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-17 |
| Detector | ADSC Q270 |
| Spacegroup name | P 62 |
| Unit cell lengths | 156.820, 156.820, 94.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.683 - 2.901 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bju |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.110 | |
| Number of reflections | 29250 | |
| <I/σ(I)> | 15.2 | 1.1 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 50 MM HEPES PH 8.0, 50 MM NACL, 7.5% PEG 4000, 1.2 MM SPERMINE |
