4UP8
Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 62 |
| Unit cell lengths | 156.483, 156.483, 93.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.541 - 2.896 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bju |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | |
| Number of reflections | 29106 | |
| <I/σ(I)> | 12.2 | 0.9 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES PH 7.5, 10% PEG 6000, 5% MPD |
