4UP7
Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase in complex with lysyl-adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 62 |
| Unit cell lengths | 155.765, 155.765, 95.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.047 - 2.789 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bju |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | |
| Number of reflections | 32784 | |
| <I/σ(I)> | 22.8 | 1.8 |
| Completeness [%] | 99.5 | 96.9 |
| Redundancy | 22.8 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50 MM HEPES PH 8.0, 50 MM NACL, 1 MM SPERMINE, 8% PEG 4000 |
