4UOZ
beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 nucleophile mutant E324A in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-26 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.200, 167.970, 108.580 |
| Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.300 |
| R-factor | 0.18926 |
| Rwork | 0.186 |
| R-free | 0.24813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uni |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.811 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.160 | 0.780 |
| Number of reflections | 95853 | |
| <I/σ(I)> | 9.64 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MGCL2, 0.1 M HEPES PH 7.5, 30% PEG 400 RESERVOIR, 0.1 UL DROP SET UP WITH A 1:1 RATIO OF RESERVOIR TO STOCK SOLUTION CONTAINING 12 MG/ML PROTEIN AND 150 MM 6-GALACTOBIOSE. |
