4UOY

Crystal structure of YgjG in complex with Pyridoxal-5'-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-03
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	120.945, 129.319, 131.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.746 - 2.305
R-factor	0.1818
Rwork	0.181
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vef
RMSD bond length	0.009
RMSD bond angle	1.136
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.360
Number of reflections	91029
<I/σ(I)>	13.4	3
Completeness [%]	98.3	96.9
Redundancy	6.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.1 M HEPES PH 7.5 15% PEG 3350 0.2 M SODIUM FORMATE 0.1 MM N-DODECYL-N,N-DIMETHYLGLYCINE