4UOW
Crystal structure of the titin M10-Obscurin Ig domain 1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-12 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 201.560, 201.560, 183.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.430 - 3.300 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wp3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.220 | 0.520 |
| Number of reflections | 64369 | |
| <I/σ(I)> | 4.3 | 2.2 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 3.6 | 291 | 3.0 M SODIUM CHLORIDE 0.1 M TRI-SODIUM CITRATE PH 3.6 BATCH SETUP |
