4UOU
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) - apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.631, 140.218, 78.791 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 78.740 - 2.400 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4agi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.610 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.740 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.110 |
| Number of reflections | 40256 | |
| <I/σ(I)> | 12.9 | 8.7 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M CACL2, 20% PEG4K, 0.1M TRIS, PH 8.5 |
