4UOP
Crystal structure of the lipoteichoic acid synthase LtaP from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.198, 53.700, 85.037 |
| Unit cell angles | 71.63, 76.78, 65.12 |
Refinement procedure
| Resolution | 80.200 - 1.750 |
| R-factor | 0.17971 |
| Rwork | 0.178 |
| R-free | 0.20738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w5q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 77013 | |
| <I/σ(I)> | 10.7 | 2.5 |
| Completeness [%] | 94.8 | 92.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 100MM NA CACODYLATE PH 5.4, 100MM MGCL2, 33% PEG2000 |
