4UO5

Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin in complex with 3SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	167.166, 349.151, 96.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	174.580 - 2.700
R-factor	0.19872
Rwork	0.197
R-free	0.22929
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uo4
RMSD bond length	0.005
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.060	0.610
Number of reflections	75251
<I/σ(I)>	19.2	3.3
Completeness [%]	97.1	98
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1