4UNT
Induced monomer of the Mcg variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-31 |
| Detector | PILATUS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.130, 90.350, 99.170 |
| Unit cell angles | 90.00, 118.86, 90.00 |
Refinement procedure
| Resolution | 63.880 - 2.700 |
| R-factor | 0.24557 |
| Rwork | 0.243 |
| R-free | 0.28925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.451 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 20681 | |
| <I/σ(I)> | 13 | 4.3 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.5M LICL, 1.65M (NH4)2SO4, pH 8 |
