4UNL
Crystal structure of a single mutant (N71D) of triosephosphate isomerase from human
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-07-28 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.943, 72.841, 93.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.280 - 1.500 |
R-factor | 0.2001 |
Rwork | 0.199 |
R-free | 0.22564 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jk2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.025 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (FOR MR) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.280 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.490 |
Number of reflections | 71346 | |
<I/σ(I)> | 7.2 | 2.5 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | PROTEIN WAS CRYSTALLIZED FROM: 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 30% W/V POLYETHYLENE GLYCOL 4,000 |