4UN3
Crystal structure of Cas9 bound to PAM-containing DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-28 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.723, 68.142, 188.230 |
| Unit cell angles | 90.00, 111.17, 90.00 |
Refinement procedure
| Resolution | 48.154 - 2.593 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.779 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.110 | 0.750 |
| Number of reflections | 126432 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 99.3 | 93.5 |
| Redundancy | 6.8 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-ACETATE PH 8.5, 0.3 M KSCN, 15% (W/V) PEG 3350 |
