4UMS
The crystal structure of the seventh ScaB type I cohesin from Pseudobacteroides cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.382, 52.767, 90.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.580 - 1.840 |
| R-factor | 0.19304 |
| Rwork | 0.190 |
| R-free | 0.25647 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ccl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.878 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.160 | 1.010 |
| Number of reflections | 13249 | |
| <I/σ(I)> | 8.9 | 1.3 |
| Completeness [%] | 99.9 | 9.9 |
| Redundancy | 7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M SODIUM CHLORIDE AND 20 % W/V PEG 3350. 30% GLYCEROL ADDED AS CRYOPROTECTANT., pH 7 |
