4UMK
The complex of Spo0J and parS DNA in chromosomal partition system
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.475, 232.731, 78.342 |
| Unit cell angles | 90.00, 109.15, 90.00 |
Refinement procedure
| Resolution | 23.574 - 3.096 |
| R-factor | 0.2539 |
| Rwork | 0.251 |
| R-free | 0.29920 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.029 |
| RMSD bond angle | 1.876 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 33332 | |
| <I/σ(I)> | 17 | 3.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PH 8 |
