4UMI
Crystal structure of the fiber head domain of the Atadenovirus snake adenovirus 1, native, F23 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 121.520, 121.520, 121.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.960 - 1.330 |
| R-factor | 0.11848 |
| Rwork | 0.117 |
| R-free | 0.13804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4D0V CHAIN A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.614 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.360 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 33985 | |
| <I/σ(I)> | 18.5 | 2.2 |
| Completeness [%] | 99.5 | 95.3 |
| Redundancy | 5.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10 MM TRIS-HCL, 1.7 M AMMONIUM SULFATE, 0.085 M HEPES SODIUM SALT PH 7.5, 1.7%(V/V) POLYETHYLENE GLYCOL (PEG) 400, 15%(V/V) GLYCEROL |
