4UM2
Crystal structure of the TPR domain of SMG6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-12 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.080, 80.676, 124.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.200 - 2.100 |
| R-factor | 0.2078 |
| Rwork | 0.206 |
| R-free | 0.23710 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 37397 | |
| <I/σ(I)> | 13.8 | 2.9 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 50MM MES PH 5.5, 200MM AMMONIUM ACETATE, 2.5% 2-METHYL-2,4-PENTANEDIOL |
