4UJ6
Structure of surface layer protein SbsC, domains 1-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.230, 98.280, 109.260 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 19.910 - 3.400 |
| R-factor | 0.18954 |
| Rwork | 0.186 |
| R-free | 0.25289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uic |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.001 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.110 | 0.430 |
| Number of reflections | 16664 | |
| <I/σ(I)> | 14.8 | 5.1 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
