4UIJ

Crystal structure of the BTB domain of KCTD13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-23
Detector	DECTRIS PIXEL
Spacegroup name	P 1 21 1
Unit cell lengths	49.450, 58.830, 87.170
Unit cell angles	90.00, 105.41, 90.00

Refinement procedure

Resolution	37.417 - 2.700
R-factor	0.2373
Rwork	0.235
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UES
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.420	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.070
Number of reflections	13431
<I/σ(I)>	8.3	2
Completeness [%]	99.8	99.8
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		1.6M MAGNESIUM SULFATE, 0.1M MES PH 6.5