4UII
Crystal structure of the Azotobacter vinelandii globin-coupled oxygen sensor in the aquo-met form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.890, 49.490, 62.770 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 30.461 - 2.827 |
| R-factor | 0.2545 |
| Rwork | 0.253 |
| R-free | 0.29670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uiq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.971 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.980 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.090 | 0.050 |
| Number of reflections | 6845 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 95.8 | 97.7 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 33% PEG 6000, 10 MM NA-CITRATE |
