4UIG
Structure of the copper sensitive operon repressor from Streptomyces lividans at pH6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 89.740, 89.740, 103.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.900 - 2.000 |
| R-factor | 0.21198 |
| Rwork | 0.211 |
| R-free | 0.23364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4adz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.730 |
| Number of reflections | 14603 | |
| <I/σ(I)> | 15.7 | 2.3 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.01 M MAGNESIUM SULFATE, 0.05 M SODIUM CACODYLATE PH 6.0, 1.8 M LITHIUM SULFATE |
