4UID

Crystal structure of the S-layer protein SbsC domains 4 and 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-29
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	110.367, 110.367, 87.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.930 - 2.800
R-factor	0.2262
Rwork	0.223
R-free	0.28636
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	POLYALA OF RSBSC AA31-844 STRUCTURE
RMSD bond length	0.011
RMSD bond angle	1.559
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.800
Rmerge	0.150
Number of reflections	14641
<I/σ(I)>	10.8
Completeness [%]	89.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				5.5 MG/ML IN 50MM TRIS/HCL PH 7.2 INDEX CONDITION NO.40 (0.01M CITRIC ACID PH 3.5, 3.0%(W/V) PEG 3350)