4UIC
Crystal structure of the S-layer protein rSbsC(31-844)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.400, 98.520, 108.584 |
| Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
| Resolution | 49.260 - 2.900 |
| R-factor | 0.23219 |
| Rwork | 0.230 |
| R-free | 0.27400 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE-RESOLVE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | |
| Number of reflections | 23384 | |
| <I/σ(I)> | 12.1 | 3 |
| Completeness [%] | 98.5 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 MG/ML 5-8% /W/V) PEG6000 FROM FLUKA, 0.05-0.2M NACL, 0.1 M CACODYLATE PH 5-6.5 |
