4UHV
The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 |
| Unit cell lengths | 78.620, 78.620, 430.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.730 - 2.000 |
| R-factor | 0.22457 |
| Rwork | 0.223 |
| R-free | 0.24845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mtk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.662 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.060 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.250 | 0.750 |
| Number of reflections | 111022 | |
| <I/σ(I)> | 6 | 1.1 |
| Completeness [%] | 91.9 | 56.1 |
| Redundancy | 12.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | PROTEIN WAS CRYSTALLIZED FROM 0.1 M HEPES PH 7.2, 0.2 M LITHIUM SULPHATE AND 38 % MPD; THEN THE CRYSTAL WAS SOAKED IN 2 MM NEODYMIUM (III) SULFATE |
