4UHS
Crystal structure of the receiver domain of CpxR from E. coli (tetragonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 114.222, 114.222, 356.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.090 - 5.000 |
| R-factor | 0.2613 |
| Rwork | 0.261 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uhj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.090 | 5.590 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Rmerge | 0.140 | |
| Number of reflections | 5469 | |
| <I/σ(I)> | 9.4 | 1.3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
