4UHS

Crystal structure of the receiver domain of CpxR from E. coli (tetragonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 41 2 2
Unit cell lengths	114.222, 114.222, 356.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.090 - 5.000
R-factor	0.2613
Rwork	0.261
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uhj
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	5.590
High resolution limit [Å]	5.000	5.000
Rmerge	0.140
Number of reflections	5469
<I/σ(I)>	9.4	1.3
Completeness [%]	99.8	99.8
Redundancy	6.9	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1