4UG1
GpsB N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 39.186, 39.186, 164.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.593 - 1.600 |
| R-factor | 0.1783 |
| Rwork | 0.174 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wuj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.888 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.850 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 20420 | |
| <I/σ(I)> | 21.9 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES 0.1M IMIDAZOLE 10% PEG 20K 20% PEG 550 MME 0.02M ALCOHOL MIX (AS PER MOLECULAR DIMESIONS MORPHEUS SCREEN), PH 6.5 |
