4UFM
Mouse Galactocerebrosidase complexed with 1-deoxy-galacto-nojirimycin DGJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 248.610, 248.610, 77.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.152 - 2.400 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zr5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.802 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.140 | 1.100 |
| Number of reflections | 35589 | |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 8.6 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE (PH 6.8), 34% PEG 8000 |
