4UF5
Crystal structure of UCH-L5 in complex with inhibitory fragment of INO80G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 95.302, 95.302, 132.576 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.300 - 3.700 |
| R-factor | 0.31184 |
| Rwork | 0.307 |
| R-free | 0.35691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.728 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 4.050 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.210 | 0.730 |
| Number of reflections | 7004 | |
| <I/σ(I)> | 7.4 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 100 MM TRIS PH 9.0, 200 MM LITHIUM CHLORIDE, 17% PEG 8000. 4 DEGREES CELSIUS |
