4UEX

Structure of human Saposin A at lysosomal pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-02
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	34.170, 58.690, 35.160
Unit cell angles	90.00, 93.10, 90.00

Refinement procedure

Resolution	34.120 - 1.800
R-factor	0.1864
Rwork	0.185
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dob
RMSD bond length	0.004
RMSD bond angle	0.807
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.120	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.100	1.000
Number of reflections	12753
<I/σ(I)>	11.2	1.8
Completeness [%]	98.8	97.7
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8		SITTING-DROP VAPOUR DIFFUSION: 400 NL DROP (200 NL PROTEIN AT 19.6 MG/ML IN 150 MM NACL, 50 MM TRIS PH 7.4 MIXED WITH 200 NL RESERVOIR SOLUTION) WAS EQUILIBRATED AGAINST A 80 UL RESERVOIR OF 0.2 M LITHIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.8 AND 25% W/V POLYETHYLENE GLYCOL 4000