Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 62 |
| Unit cell lengths | 128.487, 128.487, 52.806 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.330 - 2.950 |
| R-factor | 0.1918 |
| Rwork | 0.185 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.616 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP (AUTO MR) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.240 | 3.180 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 104864 | |
| <I/σ(I)> | 15.5 | 3.1 |
| Completeness [%] | 93.4 | 98.2 |
| Redundancy | 10.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 6.3 MG/ML OF LYSINE MODIFIED (ETHYLATED) PROTEIN IN 50 MM TRIS PH 8.0, 500 MM NACL, 5% GLYCEROL, 20 MM MGCL2, 2 MM IMIDAZOLE, 1 MM AMPPCP, MIXED 1:1 WITH 2.2 M AMMONIUM SULFATE, HANGING DROP, 18C |
