4UEO
Open state of galactitol-1-phosphate 5-dehydrogenase from E. coli, with zinc in the catalytic site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.306, 76.906, 108.651 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 44.235 - 2.000 |
| R-factor | 0.1975 |
| Rwork | 0.194 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.230 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 47058 | |
| <I/σ(I)> | 8.7 | 1.8 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
