4UEM
UCH-L5 in complex with the RPN13 DEUBAD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.596, 97.067, 100.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.853 - 2.820 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.735 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.540 | 2.970 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.060 | 0.860 |
| Number of reflections | 13635 | |
| <I/σ(I)> | 15.3 | 2.2 |
| Completeness [%] | 98.5 | 97.3 |
| Redundancy | 3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 100 MM BIS-TRIS-PROPANE PH 6.4, 230 MM NABR, 21% PEG3350. 4 DEGREES CELSIUS |
