4UEM

UCH-L5 in complex with the RPN13 DEUBAD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-25
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	56.596, 97.067, 100.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.853 - 2.820
R-factor	0.234
Rwork	0.232
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ihr
RMSD bond length	0.003
RMSD bond angle	0.735
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.540	2.970
High resolution limit [Å]	2.820	2.820
Rmerge	0.060	0.860
Number of reflections	13635
<I/σ(I)>	15.3	2.2
Completeness [%]	98.5	97.3
Redundancy	3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	100 MM BIS-TRIS-PROPANE PH 6.4, 230 MM NABR, 21% PEG3350. 4 DEGREES CELSIUS