4UEK
Galactitol-1-phosphate 5-dehydrogenase from E. coli with Tris within the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.568, 78.896, 68.572 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 45.455 - 1.900 |
| R-factor | 0.2047 |
| Rwork | 0.202 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 49264 | |
| <I/σ(I)> | 7.9 | 2.3 |
| Completeness [%] | 89.7 | 89.7 |
| Redundancy | 6.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
