4UEA

Complex of D. melanogaster eIF4E with a designed 4E-binding protein (Form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 61
Unit cell lengths	94.540, 94.540, 131.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.270 - 2.620
R-factor	0.2081
Rwork	0.205
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UE8 CHAIN A
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.690
High resolution limit [Å]	2.620	2.620
Number of reflections	20025
<I/σ(I)>	14.4	2.1
Completeness [%]	99.6	99.5
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.03M EACH DIETHYLENE GLYCOL, TRIETHYLENE GLYCOL, TETRAETHYLENE GLYCOL, PENTAETHYLENE GLYCOL, 0.1M BICINE/TRIZMA BASE PH 8.5, 10% PEG8000, 20% ETHYLENE GLYCOL