4UDT
T cell receptor (TRAV22,TRBV7-9) structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.071, 79.747, 69.035 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 39.910 - 1.350 |
| R-factor | 0.15752 |
| Rwork | 0.156 |
| R-free | 0.19291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ial 2DX9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.430 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 87661 | |
| <I/σ(I)> | 20.2 | 3.5 |
| Completeness [%] | 97.1 | 89.5 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 22% W/V PEG 2000 MME, 0.1 M NH4CL PH 8.5, 0.1 M NACL |
