4UDN
structure of metal-free periplasmic metal binding protein from candidatus liberibacter asiaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-30 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.530, 94.530, 94.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.300 - 2.210 |
| R-factor | 0.18594 |
| Rwork | 0.184 |
| R-free | 0.22386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cl2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.180 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.140 | 0.670 |
| Number of reflections | 23857 | |
| <I/σ(I)> | 10 | 3.4 |
| Completeness [%] | 96.3 | 85.4 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M SODIUM ACETATE TRIHYDRATE PH 4.6, 2M AMMONIUM SULPHATE |
