4UDG
Crystal structure of b-1,4-mannopyranosyl-chitobiose phosphorylase at 1.60 Angstrom in complex with N-acetylglucosamine and inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.816, 140.912, 168.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.120 - 1.600 |
| R-factor | 0.15507 |
| Rwork | 0.154 |
| R-free | 0.18297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.890 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 1.100 |
| Number of reflections | 261651 | |
| <I/σ(I)> | 14.49 | 2.07 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 9.37 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 17.5% PEG 3350, 200 MM NH4CL, PH 7.0 |
