4UD1
Structure of the N Terminal domain of the MERS CoV nucleocapsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-24 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.648, 67.021, 60.940 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 48.260 - 2.480 |
| R-factor | 0.2308 |
| Rwork | 0.228 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | PHENIX |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 2.480 |
| High resolution limit [Å] | 2.480 | 2.400 |
| Rmerge | 0.140 | 0.630 |
| Number of reflections | 32509 | |
| <I/σ(I)> | 5.23 | 1.9 |
| Completeness [%] | 98.0 | 97.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | NAACETATE 0.1M, AMS04 2M, pH 5 |
