4UCZ

X-ray structure and activities of an essential Mononegavirales L- protein domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-20
Detector	DECTRIS PIXEL
Spacegroup name	P 21 21 21
Unit cell lengths	78.800, 83.710, 182.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.100 - 2.990
R-factor	0.1992
Rwork	0.197
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	xia2
Data scaling software	xia2
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	78.800
High resolution limit [Å]	2.990
Rmerge	0.060
Number of reflections	25194
<I/σ(I)>	45.2
Completeness [%]	100.0
Redundancy	21.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1