4UCV

Fragment bound to H.influenza NAD dependent DNA ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-12
Detector	RIGAKU CCD
Spacegroup name	P 43 21 2
Unit cell lengths	139.160, 139.160, 56.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 2.600
R-factor	0.258
Rwork	0.257
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.980
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.550
Number of reflections	17356
<I/σ(I)>	8	2.5
Completeness [%]	98.0	98
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1